The aim of this study was to explore cross-cultural differences in symptoms of burnout, anxiety, depression, general psychological distress, and secondary traumatic stress between Asian (Japan) and European (Switzerland) midwives. One hundred seventy midwives participated in the study. There were significant differences in age group [χ ² (3) = 24.2, <i>p</i> < 0.01], marital or relationship status [χ ² (2) = 28.4, <i>p</i> < 0.01], and years of experience [χ ² (2) = 17.8, <i>p</i> < 0.01] between the two countries. The Japanese staff were younger, more often unmarried, and had less experience than the Swiss staff. The mean score of depersonalization was significantly higher in Switzerland (4.8 ± 3.8) than in Japan (3.2 ± 3.7; | <i>z</i> | = 2.71, <i>p</i> < 0.01). The mean score of general psychological distress in the Swiss sample (12.8 ± 6.5) was significantly higher than that in the Japanese sample (10.3 ± 6.2; | <i>z</i> | = 2.04, <i>p</i> = 0.04). In addition, the mean score of secondary traumatic stress was higher in the Swiss sample (31.8 ± 9.7) than in the Japanese sample (24.1 ± 8.6; | <i>z</i> | = 4.56, <i>p</i> < 0.01). These results may reflect cultural differences such as working conditions or family environment between Japan and Switzerland.