Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism
Details
Serval ID
serval:BIB_8AA38C69EF71
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism
Journal
Journal of Immunology
ISSN
0022-1767 (Print)
Publication state
Published
Issued date
08/2005
Volume
175
Number
4
Pages
2237-43
Notes
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Aug 15
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Aug 15
Abstract
Vesicle transport is a fundamental mechanism of communication in the CNS. In this study we characterized a novel type of vesicle released by murine brain microglial cells: microglial exosomes. Analysis of their protein content identified several enzymes, chaperones, tetraspanins, and membrane receptors previously reported in B cells and dendritic cell-derived exosomes. Additionally, microglia-derived exosomes expressed the aminopeptidase CD13 and the lactate transporter MCT-1. Exosomal CD13 was metabolically active in cleaving leucine- and methionine-enkephalins peptides by releasing the N-terminal tyrosine. Cleaved neuropeptides were unable to bind to the neuronal opioid receptor as assessed by cAMP response. Microglial exosomal vesicles may represent an important, previously unrecognized, cellular communication system in an organ in which cell motility is highly restricted.
Keywords
Animals
Antigens, CD13/metabolism/*physiology
Antigens, Differentiation, B-Lymphocyte/biosynthesis
Biological Markers/analysis
Cathepsins/biosynthesis
Cell Communication/immunology
Cell Line
Cell Line, Tumor
Cytoplasmic Vesicles/*enzymology/metabolism/secretion/ultrastructure
Endosomes/chemistry
Histocompatibility Antigens Class II/biosynthesis
Mice
Microglia/*enzymology/metabolism/secretion
Neuropeptides/*metabolism
Proteome/*metabolism/secretion
Pubmed
Web of science
Create date
25/01/2008 15:16
Last modification date
20/08/2019 14:49