Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism

Détails

ID Serval
serval:BIB_8AA38C69EF71
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism
Périodique
Journal of Immunology
Auteur(s)
Potolicchio  I., Carven  G. J., Xu  X., Stipp  C., Riese  R. J., Stern  L. J., Santambrogio  L.
ISSN
0022-1767 (Print)
Statut éditorial
Publié
Date de publication
08/2005
Volume
175
Numéro
4
Pages
2237-43
Notes
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Aug 15
Résumé
Vesicle transport is a fundamental mechanism of communication in the CNS. In this study we characterized a novel type of vesicle released by murine brain microglial cells: microglial exosomes. Analysis of their protein content identified several enzymes, chaperones, tetraspanins, and membrane receptors previously reported in B cells and dendritic cell-derived exosomes. Additionally, microglia-derived exosomes expressed the aminopeptidase CD13 and the lactate transporter MCT-1. Exosomal CD13 was metabolically active in cleaving leucine- and methionine-enkephalins peptides by releasing the N-terminal tyrosine. Cleaved neuropeptides were unable to bind to the neuronal opioid receptor as assessed by cAMP response. Microglial exosomal vesicles may represent an important, previously unrecognized, cellular communication system in an organ in which cell motility is highly restricted.
Mots-clé
Animals Antigens, CD13/metabolism/*physiology Antigens, Differentiation, B-Lymphocyte/biosynthesis Biological Markers/analysis Cathepsins/biosynthesis Cell Communication/immunology Cell Line Cell Line, Tumor Cytoplasmic Vesicles/*enzymology/metabolism/secretion/ultrastructure Endosomes/chemistry Histocompatibility Antigens Class II/biosynthesis Mice Microglia/*enzymology/metabolism/secretion Neuropeptides/*metabolism Proteome/*metabolism/secretion
Pubmed
Web of science
Création de la notice
25/01/2008 15:16
Dernière modification de la notice
20/08/2019 14:49
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