Effects of detergents on proliferation and metabolism of human keratinocytes

Details

Serval ID
serval:BIB_8A914BA5924D
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Effects of detergents on proliferation and metabolism of human keratinocytes
Journal
Experimental Dermatology
Author(s)
Bigliardi  P. L., Herron  M. J., Nelson  R. D., Dahl  M. V.
ISSN
0906-6705 (Print)
Publication state
Published
Issued date
04/1994
Volume
3
Number
2
Pages
89-94
Notes
Journal Article
Research Support, U.S. Gov't, P.H.S. --- Old month value: Apr
Abstract
Effects of low concentrations of detergents on cultured human foreskin keratinocytes were assessed in vitro. The viability and activity of keratinocytes were assessed by measuring reduction of a tetrazolium dye as an indicator of mitochondrial metabolism. The keratinocyte proliferative responses after incubation with detergents were assessed by a spectrophotometric assay employing crystal violet dye and a fluorometric assay determining total DNA content. Both the cationic detergent cetyltrimethylammonium bromide [CTAB] and the anionic detergent sodium lauryl sulfate [SLS] showed toxic effects on keratinocytes at concentrations as low as 3 micrograms/mg, but SLS was less toxic. However, both SLS and CTAB activated keratinocytes at very low concentrations. Proliferative activity and mitochondrial metabolism increased. Serum partially protected keratinocytes against toxic and stimulatory activities of both detergents. We suggest that detergents may directly damage keratinocytes and thereby produce irritant contact dermatitis, but activation of keratinocytes by low concentrations may also produce dermatitis, perhaps by causing keratinocytes to release cytokines.
Keywords
Cell Division/drug effects/physiology Cells, Cultured Cetrimonium Compounds/pharmacology DNA/analysis/genetics Detergents/*pharmacology Humans Infant Keratinocytes/chemistry/*cytology/*metabolism Male Mitochondria/metabolism Octoxynol/pharmacology Sodium Dodecyl Sulfate/pharmacology
Pubmed
Create date
25/01/2008 16:30
Last modification date
20/08/2019 14:49
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