Effects of detergents on proliferation and metabolism of human keratinocytes

Détails

ID Serval
serval:BIB_8A914BA5924D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Effects of detergents on proliferation and metabolism of human keratinocytes
Périodique
Experimental Dermatology
Auteur⸱e⸱s
Bigliardi  P. L., Herron  M. J., Nelson  R. D., Dahl  M. V.
ISSN
0906-6705 (Print)
Statut éditorial
Publié
Date de publication
04/1994
Volume
3
Numéro
2
Pages
89-94
Notes
Journal Article
Research Support, U.S. Gov't, P.H.S. --- Old month value: Apr
Résumé
Effects of low concentrations of detergents on cultured human foreskin keratinocytes were assessed in vitro. The viability and activity of keratinocytes were assessed by measuring reduction of a tetrazolium dye as an indicator of mitochondrial metabolism. The keratinocyte proliferative responses after incubation with detergents were assessed by a spectrophotometric assay employing crystal violet dye and a fluorometric assay determining total DNA content. Both the cationic detergent cetyltrimethylammonium bromide [CTAB] and the anionic detergent sodium lauryl sulfate [SLS] showed toxic effects on keratinocytes at concentrations as low as 3 micrograms/mg, but SLS was less toxic. However, both SLS and CTAB activated keratinocytes at very low concentrations. Proliferative activity and mitochondrial metabolism increased. Serum partially protected keratinocytes against toxic and stimulatory activities of both detergents. We suggest that detergents may directly damage keratinocytes and thereby produce irritant contact dermatitis, but activation of keratinocytes by low concentrations may also produce dermatitis, perhaps by causing keratinocytes to release cytokines.
Mots-clé
Cell Division/drug effects/physiology Cells, Cultured Cetrimonium Compounds/pharmacology DNA/analysis/genetics Detergents/*pharmacology Humans Infant Keratinocytes/chemistry/*cytology/*metabolism Male Mitochondria/metabolism Octoxynol/pharmacology Sodium Dodecyl Sulfate/pharmacology
Pubmed
Création de la notice
25/01/2008 17:30
Dernière modification de la notice
20/08/2019 15:49
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