Variability of response to methadone: genome-wide DNA methylation analysis in two independent cohorts.
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State: Public
Version: author
Serval ID
serval:BIB_8A903190C0C4
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Variability of response to methadone: genome-wide DNA methylation analysis in two independent cohorts.
Journal
Epigenomics
ISSN
1750-192X (Electronic)
ISSN-L
1750-192X
Publication state
Published
Issued date
02/2016
Peer-reviewed
Oui
Volume
8
Number
2
Pages
181-195
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Abstract
Methadone maintenance treatment is characterized by large interindividual dose variability. The aim of this study was to evaluate whether DNA methylations are associated with daily dose of methadone.
Subjects stabilized at high (n = 12) or low (n = 12) methadone doses were selected from two independent cohorts (French and Swiss). DNA methylation patterns were analyzed using HumanMethylation450 BeadChips.
In total, 584 differentially methylated sites were identified in the French cohort corresponding to 352 genes. Of these, 26 were replicated in the Swiss cohort. The methylation status of 13 genes varied similarly in both cohorts and calcium signaling pathway was significantly enriched.
Our results indicate that differentially methylated sites are associated with methadone daily dose and give insights into the molecular pathways underlying this interindividual dose variability.
Subjects stabilized at high (n = 12) or low (n = 12) methadone doses were selected from two independent cohorts (French and Swiss). DNA methylation patterns were analyzed using HumanMethylation450 BeadChips.
In total, 584 differentially methylated sites were identified in the French cohort corresponding to 352 genes. Of these, 26 were replicated in the Swiss cohort. The methylation status of 13 genes varied similarly in both cohorts and calcium signaling pathway was significantly enriched.
Our results indicate that differentially methylated sites are associated with methadone daily dose and give insights into the molecular pathways underlying this interindividual dose variability.
Keywords
Analgesics, Opioid/administration & dosage, Analgesics, Opioid/pharmacology, Cluster Analysis, Cohort Studies, CpG Islands, DNA Methylation/drug effects, Female, Gene Expression Regulation, Genome, Human, Genome-Wide Association Study, High-Throughput Nucleotide Sequencing, Humans, Male, Methadone/administration & dosage, Methadone/pharmacology
Pubmed
Create date
08/02/2016 11:24
Last modification date
20/08/2019 15:49
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