A liposomal peptide vaccine inducing CD8+ T cells in HLA-A2.1 transgenic mice, which recognise human cells encoding hepatitis C virus (HCV) proteins
Details
Serval ID
serval:BIB_834DC6847A73
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
A liposomal peptide vaccine inducing CD8+ T cells in HLA-A2.1 transgenic mice, which recognise human cells encoding hepatitis C virus (HCV) proteins
Journal
Vaccine
ISSN
0264-410X (Print)
Publication state
Published
Issued date
11/2004
Volume
23
Number
1
Pages
58-68
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Nov 15
Research Support, Non-U.S. Gov't --- Old month value: Nov 15
Abstract
Virus specific T cell responses play an important role in resolving acute hepatitis C virus (HCV) infections. Using the HLA-A2.1 transgenic mouse model we investigated the potential of a liposomal peptide vaccine to prime a CD8(+) T cell response against 10 different HCV epitopes, relevant for human applications. We were able to demonstrate the induction of strong cytotoxic T cell responses and high numbers of IFN-gamma-secreting cells, which persisted at high levels for at least 3 months. Co-integrating CpG oligonucleotides into liposomes further increased the number of IFN-gamma-secreting cells by 2-10-fold for most epitopes tested. The frequency of specific cells was further analysed with chimeric A2 tetramers bearing the NS31073-1081 epitope and was estimated at 2-23% of the CD8(+) T cell population. Importantly, mouse effector cells, specific for this epitope, were also capable of lysing a human target cell line expressing HCV proteins. This finding and the specific protection observed in challenge experiments with recombinant vaccinia virus expressing HCV sequences emphasise the biological relevance of the vaccine-induced immune response. In conclusion, such liposome formulations represent a safe and promising strategy to stimulate the CD8(+) T cell against HCV.
Keywords
Animals
CD8-Positive T-Lymphocytes/*immunology
Cell Line
HLA-A2 Antigen/genetics/*immunology
Hepacivirus/genetics/*immunology
Hepatitis, Animal/*immunology
Humans
Liposomes
Mice
Mice, Transgenic
Vaccines, Subunit/*immunology
Vaccines, Synthetic/immunology
Vaccinia/genetics
Pubmed
Web of science
Create date
25/01/2008 16:05
Last modification date
20/08/2019 14:43