A liposomal peptide vaccine inducing CD8+ T cells in HLA-A2.1 transgenic mice, which recognise human cells encoding hepatitis C virus (HCV) proteins

Détails

ID Serval
serval:BIB_834DC6847A73
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
A liposomal peptide vaccine inducing CD8+ T cells in HLA-A2.1 transgenic mice, which recognise human cells encoding hepatitis C virus (HCV) proteins
Périodique
Vaccine
Auteur⸱e⸱s
Engler  O. B., Schwendener  R. A., Dai  W. J., Wolk  B., Pichler  W., Moradpour  D., Brunner  T., Cerny  A.
ISSN
0264-410X (Print)
Statut éditorial
Publié
Date de publication
11/2004
Volume
23
Numéro
1
Pages
58-68
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Nov 15
Résumé
Virus specific T cell responses play an important role in resolving acute hepatitis C virus (HCV) infections. Using the HLA-A2.1 transgenic mouse model we investigated the potential of a liposomal peptide vaccine to prime a CD8(+) T cell response against 10 different HCV epitopes, relevant for human applications. We were able to demonstrate the induction of strong cytotoxic T cell responses and high numbers of IFN-gamma-secreting cells, which persisted at high levels for at least 3 months. Co-integrating CpG oligonucleotides into liposomes further increased the number of IFN-gamma-secreting cells by 2-10-fold for most epitopes tested. The frequency of specific cells was further analysed with chimeric A2 tetramers bearing the NS31073-1081 epitope and was estimated at 2-23% of the CD8(+) T cell population. Importantly, mouse effector cells, specific for this epitope, were also capable of lysing a human target cell line expressing HCV proteins. This finding and the specific protection observed in challenge experiments with recombinant vaccinia virus expressing HCV sequences emphasise the biological relevance of the vaccine-induced immune response. In conclusion, such liposome formulations represent a safe and promising strategy to stimulate the CD8(+) T cell against HCV.
Mots-clé
Animals CD8-Positive T-Lymphocytes/*immunology Cell Line HLA-A2 Antigen/genetics/*immunology Hepacivirus/genetics/*immunology Hepatitis, Animal/*immunology Humans Liposomes Mice Mice, Transgenic Vaccines, Subunit/*immunology Vaccines, Synthetic/immunology Vaccinia/genetics
Pubmed
Web of science
Création de la notice
25/01/2008 16:05
Dernière modification de la notice
20/08/2019 14:43
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