Intercellular adhesion molecules (ICAM)-1 ICAM-2 and ICAM-3 function as counter-receptors for lymphocyte function-associated molecule 1 in human immunodeficiency virus-mediated syncytia formation
Details
Serval ID
serval:BIB_8294B44F4F80
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Intercellular adhesion molecules (ICAM)-1 ICAM-2 and ICAM-3 function as counter-receptors for lymphocyte function-associated molecule 1 in human immunodeficiency virus-mediated syncytia formation
Journal
European Journal of Immunology
ISSN
0014-2980 (Print)
Publication state
Published
Issued date
09/1994
Volume
24
Number
9
Pages
2191-5
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Sep
Research Support, Non-U.S. Gov't --- Old month value: Sep
Abstract
It has been previously demonstrated that lymphocyte function-associated molecule 1 (LFA-1) plays a major role in human immunodeficiency virus (HIV)-mediated syncytia formation. In the present study we investigated the involvement of intercellular adhesion molecule-1 (ICAM-1), ICAM-2 and ICAM-3 in the process. The ability of monoclonal antibodies (mAb) directed against ICAM-1, ICAM-2 and ICAM-3 to block syncytia was analyzed either in phytohemagglutinin (PHA)-activated lymphocytes infected in vitro with primary or laboratory strains of HIV or by coculturing a T cell line stably expressing HIV envelope with PHA-activated lymphocytes. Complete inhibition of syncytia formation was observed only by the simultaneous addition to the cell cultures of all (i.e. anti-ICAM-1, anti-ICAM-2 and anti-ICAM-3) mAb. These results indicate that the interaction between LFA-1 and ICAM is a critical step in HIV-mediated syncytia formation, and that ICAM-1, ICAM-2 and ICAM-3 are the receptor molecules for the LFA-1-dependent syncytia formation.
Keywords
Antibodies, Monoclonal/immunology
*Antigens, CD
*Antigens, Differentiation
CD4-Positive T-Lymphocytes/microbiology
Cell Adhesion Molecules/*physiology
Cells, Cultured
Flow Cytometry
Giant Cells/*immunology/microbiology
HIV-1/*physiology
Humans
Intercellular Adhesion Molecule-1
Lymphocyte Function-Associated Antigen-1/*physiology
Receptors, Immunologic/*physiology
Pubmed
Web of science
Create date
25/01/2008 15:58
Last modification date
20/08/2019 15:42