Intercellular adhesion molecules (ICAM)-1 ICAM-2 and ICAM-3 function as counter-receptors for lymphocyte function-associated molecule 1 in human immunodeficiency virus-mediated syncytia formation

Détails

ID Serval
serval:BIB_8294B44F4F80
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Intercellular adhesion molecules (ICAM)-1 ICAM-2 and ICAM-3 function as counter-receptors for lymphocyte function-associated molecule 1 in human immunodeficiency virus-mediated syncytia formation
Périodique
European Journal of Immunology
Auteur⸱e⸱s
Butini  L., De Fougerolles  A. R., Vaccarezza  M., Graziosi  C., Cohen  D. I., Montroni  M., Springer  T. A., Pantaleo  G., Fauci  A. S.
ISSN
0014-2980 (Print)
Statut éditorial
Publié
Date de publication
09/1994
Volume
24
Numéro
9
Pages
2191-5
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Sep
Résumé
It has been previously demonstrated that lymphocyte function-associated molecule 1 (LFA-1) plays a major role in human immunodeficiency virus (HIV)-mediated syncytia formation. In the present study we investigated the involvement of intercellular adhesion molecule-1 (ICAM-1), ICAM-2 and ICAM-3 in the process. The ability of monoclonal antibodies (mAb) directed against ICAM-1, ICAM-2 and ICAM-3 to block syncytia was analyzed either in phytohemagglutinin (PHA)-activated lymphocytes infected in vitro with primary or laboratory strains of HIV or by coculturing a T cell line stably expressing HIV envelope with PHA-activated lymphocytes. Complete inhibition of syncytia formation was observed only by the simultaneous addition to the cell cultures of all (i.e. anti-ICAM-1, anti-ICAM-2 and anti-ICAM-3) mAb. These results indicate that the interaction between LFA-1 and ICAM is a critical step in HIV-mediated syncytia formation, and that ICAM-1, ICAM-2 and ICAM-3 are the receptor molecules for the LFA-1-dependent syncytia formation.
Mots-clé
Antibodies, Monoclonal/immunology *Antigens, CD *Antigens, Differentiation CD4-Positive T-Lymphocytes/microbiology Cell Adhesion Molecules/*physiology Cells, Cultured Flow Cytometry Giant Cells/*immunology/microbiology HIV-1/*physiology Humans Intercellular Adhesion Molecule-1 Lymphocyte Function-Associated Antigen-1/*physiology Receptors, Immunologic/*physiology
Pubmed
Web of science
Création de la notice
25/01/2008 14:58
Dernière modification de la notice
20/08/2019 14:42
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