Role of respiratory viruses in pulmonary exacerbations in children with cystic fibrosis.
Details
Serval ID
serval:BIB_8107581D288D
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Role of respiratory viruses in pulmonary exacerbations in children with cystic fibrosis.
Journal
Journal of Cystic Fibrosis
ISSN
1873-5010 (Electronic)
ISSN-L
1569-1993
Publication state
Published
Issued date
2012
Volume
11
Number
5
Pages
433-439
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Abstract
BACKGROUND: The role of respiratory viruses in cystic fibrosis (CF) exacerbations is incompletely understood.
METHODS: Cross-sectional study of CF children with a pulmonary exacerbation. Mid-turbinate swabs were tested by a direct immunofluorescent antibody assay and a multiplex PCR panel (ResPlex II v2.0, Qiagen). Resplex II was also applied to sputum or throat swab samples. Pulmonary function tests and quality of life and severity scores were recorded. Sputum cell counts, bacterial density and cytokines were measured.
RESULTS: 26/43 (60.5%) subjects tested positive for at least one respiratory virus by any diagnostic method applied to any sample type. Virus-positive patients were younger (p=0.047), more likely to be male (p=0.029), and had higher CF clinical severity (p=0.041) and lower quality of life (physical) scores (p=0.023) but similar IL-8, neutrophil percentage and elastase levels.
CONCLUSIONS: Compared to non-viral exacerbations, viral-related exacerbations were associated with worse severity and quality of life scores but similar pulmonary inflammation.
METHODS: Cross-sectional study of CF children with a pulmonary exacerbation. Mid-turbinate swabs were tested by a direct immunofluorescent antibody assay and a multiplex PCR panel (ResPlex II v2.0, Qiagen). Resplex II was also applied to sputum or throat swab samples. Pulmonary function tests and quality of life and severity scores were recorded. Sputum cell counts, bacterial density and cytokines were measured.
RESULTS: 26/43 (60.5%) subjects tested positive for at least one respiratory virus by any diagnostic method applied to any sample type. Virus-positive patients were younger (p=0.047), more likely to be male (p=0.029), and had higher CF clinical severity (p=0.041) and lower quality of life (physical) scores (p=0.023) but similar IL-8, neutrophil percentage and elastase levels.
CONCLUSIONS: Compared to non-viral exacerbations, viral-related exacerbations were associated with worse severity and quality of life scores but similar pulmonary inflammation.
Keywords
Adolescent, Age Factors, Bacteria/classification, Bacteria/isolation & purification, Bacterial Infections/diagnosis, Bacterial Infections/epidemiology, Canada/epidemiology, Child, Child, Preschool, Coinfection/epidemiology, Cross-Sectional Studies, Cystic Fibrosis/epidemiology, Cystic Fibrosis/physiopathology, Female, Humans, Male, Pharynx/microbiology, Prevalence, Quality of Life, Respiratory Function Tests, Respiratory Tract Infections/epidemiology, Respiratory Tract Infections/physiopathology, Severity of Illness Index, Sex Factors, Virus Diseases/diagnosis, Virus Diseases/epidemiology, Viruses/classification, Viruses/isolation & purification
Pubmed
Web of science
Open Access
Yes
Create date
30/06/2014 11:05
Last modification date
20/08/2019 14:41
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