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Azidothymidine (AZT) as a potential modifier of radiation response in vitro
The potential effect of AZT as a thymidine analogue on radiation response in vitro was investigated. Two human cell lines (WiDr and HeLa) were used. The effect of 10 microM AZT on exponentially growing cells was studied after different exposure times (24, 48 and 72 h). The surviving fraction (clonogenic assay) or metabolic activity (MTT assay) after irradiation of AZT-exposed cells, was compared to unexposed irradiated controls. Flow cytometry was used to assess the cell-cycle effect of pre-exposure of exponentially growing cells to AZT. AZT had a radioprotective effect for all experimental time points as far as WiDr was concerned. For HeLa the effect was significant at 24 h. Cell-cycle analysis showed a significant accumulation in S-phase at 72 h for WiDr. For HeLa there was a significant accumulation in S-phase at 48 h. We conclude that under the reported experimental conditions, AZT as a thymidine analogue seems to reduce the cytotoxic effect of irradiation.
Biological Response Modifiers Cell Cycle/drug effects/radiation effects Dose-Response Relationship, Radiation Hela Cells/drug effects/metabolism/radiation effects Humans Radiation-Protective Agents/*pharmacology Tumor Cells, Cultured/*drug effects/metabolism/*radiation effects Zidovudine/*pharmacology
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