Azidothymidine (AZT) as a potential modifier of radiation response in vitro
Détails
ID Serval
serval:BIB_807D1028431A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Azidothymidine (AZT) as a potential modifier of radiation response in vitro
Périodique
Acta Oncologica
ISSN
0284-186X (Print)
Statut éditorial
Publié
Date de publication
1995
Volume
34
Numéro
2
Pages
213-8
Notes
Comparative Study
Journal Article
Journal Article
Résumé
The potential effect of AZT as a thymidine analogue on radiation response in vitro was investigated. Two human cell lines (WiDr and HeLa) were used. The effect of 10 microM AZT on exponentially growing cells was studied after different exposure times (24, 48 and 72 h). The surviving fraction (clonogenic assay) or metabolic activity (MTT assay) after irradiation of AZT-exposed cells, was compared to unexposed irradiated controls. Flow cytometry was used to assess the cell-cycle effect of pre-exposure of exponentially growing cells to AZT. AZT had a radioprotective effect for all experimental time points as far as WiDr was concerned. For HeLa the effect was significant at 24 h. Cell-cycle analysis showed a significant accumulation in S-phase at 72 h for WiDr. For HeLa there was a significant accumulation in S-phase at 48 h. We conclude that under the reported experimental conditions, AZT as a thymidine analogue seems to reduce the cytotoxic effect of irradiation.
Mots-clé
Biological Response Modifiers
Cell Cycle/drug effects/radiation effects
Dose-Response Relationship, Radiation
Hela Cells/drug effects/metabolism/radiation effects
Humans
Radiation-Protective Agents/*pharmacology
Tumor Cells, Cultured/*drug effects/metabolism/*radiation effects
Zidovudine/*pharmacology
Pubmed
Web of science
Création de la notice
24/01/2008 18:12
Dernière modification de la notice
20/08/2019 15:41