Article: article from journal or magazin.
Macrophage NO2- production as a sensitive and rapid assay for the quantitation of murine IFN-gamma
Journal of Immunological Methods
Journal Article --- Old month value: May 17
Macrophage production of arginine-derived NO2- provides a simple method for detection and quantitation of interferon-gamma (IFN-gamma) in murine cell culture fluids. When the macrophage cell line RAW 264 is cultured overnight with IFN-gamma in the presence of 10 ng/ml LPS, NO2- release, as determined by a simple colorimetric assay, is proportional to the concentration of IFN-gamma and is inhibited by monoclonal antibody to IFN-gamma. A high degree of correlation was obtained when antigen stimulated T lymphocyte supernatants were tested for IFN-gamma by ELISA and the NO2- production assay.
Animals Biological Assay Cell Line Dose-Response Relationship, Drug Enzyme-Linked Immunosorbent Assay Interferon Type II/*analysis/pharmacology Lipopolysaccharides/pharmacology Macrophages/drug effects/*metabolism Mice Mice, Inbred BALB C Nitrites/*metabolism Transforming Growth Factor beta/pharmacology
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