Macrophage NO2- production as a sensitive and rapid assay for the quantitation of murine IFN-gamma

Détails

ID Serval
serval:BIB_7D66274FA483
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Macrophage NO2- production as a sensitive and rapid assay for the quantitation of murine IFN-gamma
Périodique
Journal of Immunological Methods
Auteur⸱e⸱s
Migliorini  P., Corradin  G., Corradin  S. B.
ISSN
0022-1759 (Print)
Statut éditorial
Publié
Date de publication
05/1991
Volume
139
Numéro
1
Pages
107-14
Notes
Journal Article --- Old month value: May 17
Résumé
Macrophage production of arginine-derived NO2- provides a simple method for detection and quantitation of interferon-gamma (IFN-gamma) in murine cell culture fluids. When the macrophage cell line RAW 264 is cultured overnight with IFN-gamma in the presence of 10 ng/ml LPS, NO2- release, as determined by a simple colorimetric assay, is proportional to the concentration of IFN-gamma and is inhibited by monoclonal antibody to IFN-gamma. A high degree of correlation was obtained when antigen stimulated T lymphocyte supernatants were tested for IFN-gamma by ELISA and the NO2- production assay.
Mots-clé
Animals Biological Assay Cell Line Dose-Response Relationship, Drug Enzyme-Linked Immunosorbent Assay Interferon Type II/*analysis/pharmacology Lipopolysaccharides/pharmacology Macrophages/drug effects/*metabolism Mice Mice, Inbred BALB C Nitrites/*metabolism Transforming Growth Factor beta/pharmacology
Pubmed
Web of science
Création de la notice
24/01/2008 14:55
Dernière modification de la notice
20/08/2019 14:38
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