Induction of specific immune unresponsiveness with purified mixed leukocyte culture-activated T lymphoblasts as autoimmunogen. II. An analysis of the effects measured at the cellular and serological levels.

Details

Serval ID
serval:BIB_774B96501C47
Type
Article: article from journal or magazin.
Collection
Publications
Title
Induction of specific immune unresponsiveness with purified mixed leukocyte culture-activated T lymphoblasts as autoimmunogen. II. An analysis of the effects measured at the cellular and serological levels.
Journal
Journal of Experimental Medicine
Author(s)
Aguet M., Andersson L.C., Andersson R., Wight E., Binz H., Wigzell H.
ISSN
0022-1007 (Print)
ISSN-L
0022-1007
Publication state
Published
Issued date
1978
Volume
147
Number
1
Pages
50-61
Language
english
Abstract
T lymphoblasts specific for foreign histocompatibility antigens and purified via mixed leukocyte culture (MLC) and 1 g velocity sedimentation procedures can be used as autoimmunogen to produce specific immunological unresponsiveness in adult animals. This unresponsiveness is positively correlated to the production of autoanti-idiotypic antibodies in the blast immunized animals and no evidence of coexisting alloimmunity was found. We consider this autoanti-idiotypic immunity to be the specific inducing agent of the immune tolerance. The blast immunization procedure will lead to selective reduction in T-cell reactivity against the relevant alloantigens as measured by MLC, cell-mediated lympholysis, or graft-versus-host assays. However, in individual animals, dichtomy in suppression between two T-cell assays could sometimes be observed indicating elimination of only a select group of idiotypic functionally distinct population of T cells in these blast-immunized animals. Attempts to abrogate already immune animals by the autoblast procedure were successful, in part suggesting the use of the present procedure when trying to induce in accelerated reversion of such immunity.
Keywords
Animals, Antibodies, Anti-Idiotypic, Antigens, Autoantibodies, Autoantigens, Epitopes, Histocompatibility Antigens, Immune Tolerance, Immunoglobulins, Immunosuppression, Lymphocyte Culture Test, Mixed, Mice, Mice, Inbred Strains, Rats, Rats, Inbred Strains, T-Lymphocytes/immunology
Pubmed
Web of science
Open Access
Yes
Create date
28/01/2008 11:37
Last modification date
20/08/2019 14:34
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