Identification of the biosynthetic gene cluster for the Pseudomonas aeruginosa antimetabolite L-2-amino-4-methoxy-trans-3-butenoic acid.

Lee, X.; Fox, A.; Sufrin, J.; Henry, H.; Majcherczyk, P.; Haas, D.; Reimmann, C.

doi:10.1128/JB.00492-10

Identification of the biosynthetic gene cluster for the Pseudomonas aeruginosa antimetabolite L-2-amino-4-methoxy-trans-3-butenoic acid.

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Serval ID

serval:BIB_7561C44C2B0E

Type

Article: article from journal or magazin.

Collection

Publications

Institution

UNIL/CHUV

Title

Identification of the biosynthetic gene cluster for the Pseudomonas aeruginosa antimetabolite L-2-amino-4-methoxy-trans-3-butenoic acid.

Journal

Journal of Bacteriology

Author(s)

Lee X., Fox A., Sufrin J., Henry H., Majcherczyk P., Haas D., Reimmann C.

ISSN

1098-5530[electronic], 0021-9193[linking]

Publication state

Published

Issued date

2010

Volume

192

Number

Pages

4251-4255

Language

english

Abstract

L-2-amino-4-methoxy-trans-3-butenoic acid (AMB) is a potent antibiotic and toxin produced by Pseudomonas aeruginosa. Using a novel biochemical assay combined with site-directed mutagenesis in strain PAO1, we have identified a five-gene cluster specifying AMB biosynthesis, probably involving a thiotemplate mechanism. Overexpression of this cluster in strain PA7, a natural AMB-negative isolate, led to AMB overproduction.

Keywords

Aminobutyric Acids/metabolism, Anti-Bacterial Agents/biosynthesis, Antimetabolites/metabolism, Biosynthetic Pathways/genetics, Gene Order, Genes, Bacterial, Multigene Family, Mutagenesis, Site-Directed, Pseudomonas aeruginosa/genetics, Pseudomonas aeruginosa/metabolism

OAI-PMH

oai:serval.unil.ch:BIB_7561C44C2B0E

DOI

10.1128/JB.00492-10

Pubmed

20543073

Web of science

000280406300020

Open Access

Yes

Create date

21/01/2011 8:43