Identification of the biosynthetic gene cluster for the Pseudomonas aeruginosa antimetabolite L-2-amino-4-methoxy-trans-3-butenoic acid.
Détails
ID Serval
serval:BIB_7561C44C2B0E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Identification of the biosynthetic gene cluster for the Pseudomonas aeruginosa antimetabolite L-2-amino-4-methoxy-trans-3-butenoic acid.
Périodique
Journal of Bacteriology
ISSN
1098-5530[electronic], 0021-9193[linking]
Statut éditorial
Publié
Date de publication
2010
Volume
192
Numéro
16
Pages
4251-4255
Langue
anglais
Résumé
L-2-amino-4-methoxy-trans-3-butenoic acid (AMB) is a potent antibiotic and toxin produced by Pseudomonas aeruginosa. Using a novel biochemical assay combined with site-directed mutagenesis in strain PAO1, we have identified a five-gene cluster specifying AMB biosynthesis, probably involving a thiotemplate mechanism. Overexpression of this cluster in strain PA7, a natural AMB-negative isolate, led to AMB overproduction.
Mots-clé
Aminobutyric Acids/metabolism, Anti-Bacterial Agents/biosynthesis, Antimetabolites/metabolism, Biosynthetic Pathways/genetics, Gene Order, Genes, Bacterial, Multigene Family, Mutagenesis, Site-Directed, Pseudomonas aeruginosa/genetics, Pseudomonas aeruginosa/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
21/01/2011 9:43
Dernière modification de la notice
20/08/2019 15:32
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