Identification of the biosynthetic gene cluster for the Pseudomonas aeruginosa antimetabolite L-2-amino-4-methoxy-trans-3-butenoic acid.

Details

Serval ID
serval:BIB_7561C44C2B0E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Identification of the biosynthetic gene cluster for the Pseudomonas aeruginosa antimetabolite L-2-amino-4-methoxy-trans-3-butenoic acid.
Journal
Journal of Bacteriology
Author(s)
Lee X., Fox A., Sufrin J., Henry H., Majcherczyk P., Haas D., Reimmann C.
ISSN
1098-5530[electronic], 0021-9193[linking]
Publication state
Published
Issued date
2010
Volume
192
Number
16
Pages
4251-4255
Language
english
Abstract
L-2-amino-4-methoxy-trans-3-butenoic acid (AMB) is a potent antibiotic and toxin produced by Pseudomonas aeruginosa. Using a novel biochemical assay combined with site-directed mutagenesis in strain PAO1, we have identified a five-gene cluster specifying AMB biosynthesis, probably involving a thiotemplate mechanism. Overexpression of this cluster in strain PA7, a natural AMB-negative isolate, led to AMB overproduction.
Keywords
Aminobutyric Acids/metabolism, Anti-Bacterial Agents/biosynthesis, Antimetabolites/metabolism, Biosynthetic Pathways/genetics, Gene Order, Genes, Bacterial, Multigene Family, Mutagenesis, Site-Directed, Pseudomonas aeruginosa/genetics, Pseudomonas aeruginosa/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
21/01/2011 9:43
Last modification date
20/08/2019 15:32
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