Article: article from journal or magazin.
Monocyte attachment to activated human vascular endothelium in vitro is mediated by leukocyte adhesion molecule-1 (L-selectin) under nonstatic conditions
Journal of Experimental Medicine
Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. --- Old month value: Jun 1
The receptors that mediate monocyte adhesion to cytokine-stimulated endothelial monolayers were assessed using a nonstatic (rotating) cell-attachment assay. In this system, leukocyte adhesion molecule-1 (LAM-1) (L-selectin) mediated a major portion (87 +/- 15% at 37 degrees C) of monocyte attachment to activated endothelium. mAb blocking of endothelial leukocyte adhesion molecule-1 (41% inhibition), CD18 (36%), and vascular cell adhesion molecule-1 (25%) function had lesser effects on attachment. These results suggest that LAM-1 may serve an important role in monocyte attachment to endothelium at sites of inflammation.
Antibodies, Monoclonal Antigens, CD/physiology Antigens, CD18 *Cell Adhesion/drug effects Cell Adhesion Molecules/biosynthesis/immunology/*physiology Cell Line Endothelium, Vascular/drug effects/*physiology Humans L-Selectin Monocytes/drug effects/*physiology Receptors, Leukocyte-Adhesion/physiology Tumor Necrosis Factor-alpha/drug effects Umbilical Veins Vascular Cell Adhesion Molecule-1
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