Hyperplectonemes: A Higher Order Compact and Dynamic DNA Self-Organization.

Details

Serval ID
serval:BIB_6CDAD1F0847A
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Hyperplectonemes: A Higher Order Compact and Dynamic DNA Self-Organization.
Journal
Nano Letters
Author(s)
Japaridze A., Muskhelishvili G., Benedetti F., Gavriilidou A.F., Zenobi R., De Los Rios P., Longo G., Dietler G.
ISSN
1530-6992 (Electronic)
ISSN-L
1530-6984
Publication state
Published
Issued date
2017
Peer-reviewed
Oui
Volume
17
Number
3
Pages
1938-1948
Language
english
Abstract
Bacterial chromosome has a compact structure that dynamically changes its shape in response to bacterial growth rate and growth phase. Determining how chromatin remains accessible to DNA binding proteins, and transcription machinery is crucial to understand the link between genetic regulation, DNA structure, and topology. Here, we study very large supercoiled dsDNA using high-resolution characterization, theoretical modeling, and molecular dynamics calculations. We unveil a new type of highly ordered DNA organization forming in the presence of attractive DNA-DNA interactions, which we call hyperplectonemes. We demonstrate that their formation depends on DNA size, supercoiling, and bacterial physiology. We compare structural, nanomechanic, and dynamic properties of hyperplectonemes bound by three highly abundant nucleoid-associated proteins (FIS, H-NS, and HU). In all these cases, the negative supercoiling of DNA determines molecular dynamics, modulating their 3D shape. Overall, our findings provide a mechanistic insight into the critical role of DNA topology in genetic regulation.

Keywords
DNA, supercoiling, plectonemes, self-organization, nucleoid associated proteins, H-NS, FIS, HU
Pubmed
Web of science
Create date
21/02/2017 18:49
Last modification date
20/08/2019 14:26
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