Hyperplectonemes: A Higher Order Compact and Dynamic DNA Self-Organization.

Détails

ID Serval
serval:BIB_6CDAD1F0847A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Hyperplectonemes: A Higher Order Compact and Dynamic DNA Self-Organization.
Périodique
Nano Letters
Auteur⸱e⸱s
Japaridze A., Muskhelishvili G., Benedetti F., Gavriilidou A.F., Zenobi R., De Los Rios P., Longo G., Dietler G.
ISSN
1530-6992 (Electronic)
ISSN-L
1530-6984
Statut éditorial
Publié
Date de publication
2017
Peer-reviewed
Oui
Volume
17
Numéro
3
Pages
1938-1948
Langue
anglais
Résumé
Bacterial chromosome has a compact structure that dynamically changes its shape in response to bacterial growth rate and growth phase. Determining how chromatin remains accessible to DNA binding proteins, and transcription machinery is crucial to understand the link between genetic regulation, DNA structure, and topology. Here, we study very large supercoiled dsDNA using high-resolution characterization, theoretical modeling, and molecular dynamics calculations. We unveil a new type of highly ordered DNA organization forming in the presence of attractive DNA-DNA interactions, which we call hyperplectonemes. We demonstrate that their formation depends on DNA size, supercoiling, and bacterial physiology. We compare structural, nanomechanic, and dynamic properties of hyperplectonemes bound by three highly abundant nucleoid-associated proteins (FIS, H-NS, and HU). In all these cases, the negative supercoiling of DNA determines molecular dynamics, modulating their 3D shape. Overall, our findings provide a mechanistic insight into the critical role of DNA topology in genetic regulation.

Mots-clé
DNA, supercoiling, plectonemes, self-organization, nucleoid associated proteins, H-NS, FIS, HU
Pubmed
Web of science
Création de la notice
21/02/2017 19:49
Dernière modification de la notice
20/08/2019 15:26
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