Natural alleles at the Doa locus underpin evolutionary changes in Drosophila lifespan and fecundity.

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Version: Final published version
License: CC BY 4.0
Serval ID
serval:BIB_6B05F1D08FA1
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Natural alleles at the Doa locus underpin evolutionary changes in Drosophila lifespan and fecundity.
Journal
Proceedings. Biological sciences
Author(s)
Hoedjes K.M., Kostic H., Keller L., Flatt T.
ISSN
1471-2954 (Electronic)
ISSN-L
0962-8452
Publication state
Published
Issued date
09/11/2022
Peer-reviewed
Oui
Volume
289
Number
1986
Pages
20221989
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
'Evolve and resequence' (E&amp;R) studies in <i>Drosophila melanogaster</i> have identified many candidate loci underlying the evolution of ageing and life history, but experiments that validate the effects of such candidates remain rare. In a recent E&amp;R study we have identified several alleles of the LAMMER kinase <i>Darkener of apricot</i> (<i>Doa</i>) as candidates for evolutionary changes in lifespan and fecundity. Here, we use two complementary approaches to confirm a functional role of <i>Doa</i> in life-history evolution. First, we used transgenic RNAi to study the effects of <i>Doa</i> at the whole-gene level. Ubiquitous silencing of expression in adult flies reduced both lifespan and fecundity, indicating pleiotropic effects. Second, to characterize segregating variation at <i>Doa</i>, we examined four candidate single nucleotide polymorphisms (SNPs; <i>Doa-1</i>, -<i>2</i>, -<i>3</i>, <i>-4</i>) using a genetic association approach. Three candidate SNPs had effects that were qualitatively consistent with expectations based on our E&amp;R study: <i>Doa-2</i> pleiotropically affected both lifespan and late-life fecundity; <i>Doa-1</i> affected lifespan (but not fecundity); and <i>Doa-4</i> affected late-life fecundity (but not lifespan). Finally, the last candidate allele (<i>Doa-3</i>) also affected lifespan, but in the opposite direction from predicted.
Keywords
Animals, Drosophila melanogaster/genetics, Drosophila melanogaster/metabolism, Drosophila/genetics, Alleles, Prunus armeniaca/genetics, Prunus armeniaca/metabolism, Longevity, Drosophila Proteins/genetics, Drosophila Proteins/metabolism, Drosophila, ageing, experimental evolution, pleiotropy, reproduction
Pubmed
Open Access
Yes
Create date
16/11/2022 16:03
Last modification date
28/07/2023 6:11
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