Determination of 5,10,15,20-tetra-(m-hydroxyphenyl)chlorin in tissues by high performance liquid chromatography
Details
Serval ID
serval:BIB_696296FE8829
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Determination of 5,10,15,20-tetra-(m-hydroxyphenyl)chlorin in tissues by high performance liquid chromatography
Journal
Biomedical Chromatography
ISSN
0269-3879 (Print)
Publication state
Published
Issued date
06/1993
Volume
7
Number
3
Pages
155-7
Notes
Journal Article --- Old url value: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8318833 --- Old month value: May-Jun
Abstract
A procedure for the extraction and high performance liquid chromatographic (HPLC) determination of the photodynamic therapeutic agent 5,10,15,20-tetra(m-hydroxyphenyl)chlorin in human, rat and mouse tissues following intravenous administration of the drug is described. The tissue (tumour, skin, muscle and liver) was homogenized and extracted into a mixture of methanol:dimethyl sulphoxide:water (32:8:1 by vol.) containing, 5,10,15,20-tetra(p-hydroxyphenyl)chlorin as the internal standard. The precipitated proteins were removed by centrifugation and the supernatant was separated by reversed phase HPLC on a Hypersil-ODS column with 77% (v/v) acetonitrile in 0.1% trifluoroacetic acid as the mobile phase. The solute was detected with high sensitivity and specificity by a UV-VIS detector set at 423 nm.
Keywords
Animals
Chromatography, High Pressure Liquid/*methods
Dimethyl Sulfoxide
Humans
Liver/chemistry
Mesoporphyrins/*analysis
Methanol
Mice
Muscles/chemistry
Neoplasms/chemistry
Radiation-Sensitizing Agents/*analysis
Rats
Skin/chemistry
Pubmed
Web of science
Create date
29/01/2008 14:00
Last modification date
20/08/2019 15:24