Determination of 5,10,15,20-tetra-(m-hydroxyphenyl)chlorin in tissues by high performance liquid chromatography

Détails

ID Serval
serval:BIB_696296FE8829
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Determination of 5,10,15,20-tetra-(m-hydroxyphenyl)chlorin in tissues by high performance liquid chromatography
Périodique
Biomedical Chromatography
Auteur⸱e⸱s
Wang  Q., Altermatt  H. J., Ris  H. B., Reynolds  B. E., Stewart  J. C., Bonnett  R., Lim  C. K.
ISSN
0269-3879 (Print)
Statut éditorial
Publié
Date de publication
06/1993
Volume
7
Numéro
3
Pages
155-7
Notes
Journal Article --- Old url value: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8318833 --- Old month value: May-Jun
Résumé
A procedure for the extraction and high performance liquid chromatographic (HPLC) determination of the photodynamic therapeutic agent 5,10,15,20-tetra(m-hydroxyphenyl)chlorin in human, rat and mouse tissues following intravenous administration of the drug is described. The tissue (tumour, skin, muscle and liver) was homogenized and extracted into a mixture of methanol:dimethyl sulphoxide:water (32:8:1 by vol.) containing, 5,10,15,20-tetra(p-hydroxyphenyl)chlorin as the internal standard. The precipitated proteins were removed by centrifugation and the supernatant was separated by reversed phase HPLC on a Hypersil-ODS column with 77% (v/v) acetonitrile in 0.1% trifluoroacetic acid as the mobile phase. The solute was detected with high sensitivity and specificity by a UV-VIS detector set at 423 nm.
Mots-clé
Animals Chromatography, High Pressure Liquid/*methods Dimethyl Sulfoxide Humans Liver/chemistry Mesoporphyrins/*analysis Methanol Mice Muscles/chemistry Neoplasms/chemistry Radiation-Sensitizing Agents/*analysis Rats Skin/chemistry
Pubmed
Web of science
Création de la notice
29/01/2008 14:00
Dernière modification de la notice
20/08/2019 15:24
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