Determination of 5,10,15,20-tetra-(m-hydroxyphenyl)chlorin in tissues by high performance liquid chromatography

Details

Serval ID
serval:BIB_696296FE8829
Type
Article: article from journal or magazin.
Collection
Publications
Title
Determination of 5,10,15,20-tetra-(m-hydroxyphenyl)chlorin in tissues by high performance liquid chromatography
Journal
Biomedical Chromatography
Author(s)
Wang  Q., Altermatt  H. J., Ris  H. B., Reynolds  B. E., Stewart  J. C., Bonnett  R., Lim  C. K.
ISSN
0269-3879 (Print)
Publication state
Published
Issued date
06/1993
Volume
7
Number
3
Pages
155-7
Notes
Journal Article --- Old url value: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8318833 --- Old month value: May-Jun
Abstract
A procedure for the extraction and high performance liquid chromatographic (HPLC) determination of the photodynamic therapeutic agent 5,10,15,20-tetra(m-hydroxyphenyl)chlorin in human, rat and mouse tissues following intravenous administration of the drug is described. The tissue (tumour, skin, muscle and liver) was homogenized and extracted into a mixture of methanol:dimethyl sulphoxide:water (32:8:1 by vol.) containing, 5,10,15,20-tetra(p-hydroxyphenyl)chlorin as the internal standard. The precipitated proteins were removed by centrifugation and the supernatant was separated by reversed phase HPLC on a Hypersil-ODS column with 77% (v/v) acetonitrile in 0.1% trifluoroacetic acid as the mobile phase. The solute was detected with high sensitivity and specificity by a UV-VIS detector set at 423 nm.
Keywords
Animals Chromatography, High Pressure Liquid/*methods Dimethyl Sulfoxide Humans Liver/chemistry Mesoporphyrins/*analysis Methanol Mice Muscles/chemistry Neoplasms/chemistry Radiation-Sensitizing Agents/*analysis Rats Skin/chemistry
Pubmed
Web of science
Create date
29/01/2008 13:00
Last modification date
20/08/2019 14:24
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