Spontaneous deletions of the chromosome-mobilizing plasmid R68.45 in Pseudomonas aeruginosa PAO.

Details

Serval ID
serval:BIB_6939200214BD
Type
Article: article from journal or magazin.
Collection
Publications
Title
Spontaneous deletions of the chromosome-mobilizing plasmid R68.45 in Pseudomonas aeruginosa PAO.
Journal
Plasmid
Author(s)
Haas D., Riess G.
ISSN
0147-619X (Print)
ISSN-L
0147-619X
Publication state
Published
Issued date
1983
Volume
9
Number
1
Pages
42-52
Language
english
Abstract
In Pseudomonas aeruginosa strain PAO the chromosome-mobilizing IncP-1 plasmid R68.45 was unstable whereas the parent plasmid R68 was stable. The instability of R68.45 was observed in rec+ and rec strains within about 100 generations after conjugal transfer of the plasmid and, to a lesser extent, in established R68.45 donor strains. Two phenotypically distinct classes of R68.45 derivatives were obtained: (i) CbR (carbenicillin-resistant), TcR (tetracycline-resistant), KmR (kanamycin-resistant), Tra+ (transfer proficient), Cma- (chromosome-mobilizing ability), lacking the duplicated IS21 copy typical of R68.45 and indistinguishable from R68 by restriction enzyme analysis; (ii) CbR TcR KmS Tra- Cma-, due to deletion of one IS21 copy, the adjacent KmR gene, and a variable part of the Tra-1 region including, in most cases, the origin of transfer (oriT). Both types of deletion derivatives were stable. R68.45 derivatives lacking the Tra-2 region were not recovered spontaneously, but could be constructed in vitro and were stable in strain PAO. Deletion formation of type ii as well as Cma did not depend on homologous recombination and can be ascribed to functions of the duplicated IS21. Chromosome mobilization does not appear to require obligatory transfer of the entire R68.45 plasmid. Four ClaI restriction sites were mapped on R68 extracted from P. aeruginosa. One of these sites was cryptic, presumably because of methylation, when the plasmid was prepared from Escherichia coli (dam+).
Keywords
Chromosome Deletion, Chromosome Mapping, Chromosomes, Bacterial, Conjugation, Genetic, DNA Restriction Enzymes, Plasmids, Pseudomonas aeruginosa/genetics, Recombination, Genetic
Pubmed
Web of science
Create date
25/01/2008 17:01
Last modification date
20/08/2019 14:24
Usage data