Spontaneous deletions of the chromosome-mobilizing plasmid R68.45 in Pseudomonas aeruginosa PAO.

Détails

ID Serval
serval:BIB_6939200214BD
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Spontaneous deletions of the chromosome-mobilizing plasmid R68.45 in Pseudomonas aeruginosa PAO.
Périodique
Plasmid
Auteur⸱e⸱s
Haas D., Riess G.
ISSN
0147-619X (Print)
ISSN-L
0147-619X
Statut éditorial
Publié
Date de publication
1983
Volume
9
Numéro
1
Pages
42-52
Langue
anglais
Résumé
In Pseudomonas aeruginosa strain PAO the chromosome-mobilizing IncP-1 plasmid R68.45 was unstable whereas the parent plasmid R68 was stable. The instability of R68.45 was observed in rec+ and rec strains within about 100 generations after conjugal transfer of the plasmid and, to a lesser extent, in established R68.45 donor strains. Two phenotypically distinct classes of R68.45 derivatives were obtained: (i) CbR (carbenicillin-resistant), TcR (tetracycline-resistant), KmR (kanamycin-resistant), Tra+ (transfer proficient), Cma- (chromosome-mobilizing ability), lacking the duplicated IS21 copy typical of R68.45 and indistinguishable from R68 by restriction enzyme analysis; (ii) CbR TcR KmS Tra- Cma-, due to deletion of one IS21 copy, the adjacent KmR gene, and a variable part of the Tra-1 region including, in most cases, the origin of transfer (oriT). Both types of deletion derivatives were stable. R68.45 derivatives lacking the Tra-2 region were not recovered spontaneously, but could be constructed in vitro and were stable in strain PAO. Deletion formation of type ii as well as Cma did not depend on homologous recombination and can be ascribed to functions of the duplicated IS21. Chromosome mobilization does not appear to require obligatory transfer of the entire R68.45 plasmid. Four ClaI restriction sites were mapped on R68 extracted from P. aeruginosa. One of these sites was cryptic, presumably because of methylation, when the plasmid was prepared from Escherichia coli (dam+).
Mots-clé
Chromosome Deletion, Chromosome Mapping, Chromosomes, Bacterial, Conjugation, Genetic, DNA Restriction Enzymes, Plasmids, Pseudomonas aeruginosa/genetics, Recombination, Genetic
Pubmed
Web of science
Création de la notice
25/01/2008 17:01
Dernière modification de la notice
20/08/2019 14:24
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