(TG)n uncovers a sex-specific hybridization pattern in cattle

Details

Serval ID
serval:BIB_6677E86A54D1
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
(TG)n uncovers a sex-specific hybridization pattern in cattle
Journal
Genomics
Author(s)
Kashi  Y., Iraqi  F., Tikochinski  Y., Ruzitsky  B., Nave  A., Beckmann  J. S., Friedmann  A., Soller  M., Gruenbaum  Y.
ISSN
0888-7543 (Print)
Publication state
Published
Issued date
05/1990
Volume
7
Number
1
Pages
31-6
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S. --- Old month value: May
Abstract
Screening of a bovine genomic library with the human minisatellite 33.6 probe uncovered a family of clones that, when used to probe Southern blots of bovine genomic DNA digested with the restriction enzyme HaeIII or MboI, revealed sexually dimorphic, but otherwise virtually monomorphic, patterns among the larger DNA fragments to which they hybridized. Characterization of one of these clones revealed that it contains different minisatellite sequences. The sexual dimorphism hybridization pattern observed with this clone was found to be due to multiple copies of two tandemly interspersed repeats: the simple sequence (TG)n and a previously undescribed 29-bp sequence. Both repeats appear to share many genomic loci including autosomal loci. In contrast, Southern analysis of AluI- or HinfI-digested bovine DNA with the (TG)n repeat used as a probe yielded substantial polymorphism. These results show that (i) different minisatellites can be found in a cluster, (ii) both simple and more complex repeated sequences other than the simple quaternary (GATA)n repeat can be sexually dimorphic, and (iii) simple repeats can reveal substantial polymorphism.
Keywords
Animals Bacteriophage lambda/genetics Base Sequence Blotting, Southern Cattle Cloning, Molecular DNA, Satellite/genetics Female Humans Male Molecular Sequence Data Multigene Family Nucleic Acid Hybridization Polymorphism, Restriction Fragment Length *Repetitive Sequences, Nucleic Acid Restriction Mapping *Sex Characteristics
Pubmed
Web of science
Create date
25/01/2008 16:18
Last modification date
20/08/2019 14:22
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