(TG)n uncovers a sex-specific hybridization pattern in cattle
Détails
ID Serval
serval:BIB_6677E86A54D1
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
(TG)n uncovers a sex-specific hybridization pattern in cattle
Périodique
Genomics
ISSN
0888-7543 (Print)
Statut éditorial
Publié
Date de publication
05/1990
Volume
7
Numéro
1
Pages
31-6
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S. --- Old month value: May
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S. --- Old month value: May
Résumé
Screening of a bovine genomic library with the human minisatellite 33.6 probe uncovered a family of clones that, when used to probe Southern blots of bovine genomic DNA digested with the restriction enzyme HaeIII or MboI, revealed sexually dimorphic, but otherwise virtually monomorphic, patterns among the larger DNA fragments to which they hybridized. Characterization of one of these clones revealed that it contains different minisatellite sequences. The sexual dimorphism hybridization pattern observed with this clone was found to be due to multiple copies of two tandemly interspersed repeats: the simple sequence (TG)n and a previously undescribed 29-bp sequence. Both repeats appear to share many genomic loci including autosomal loci. In contrast, Southern analysis of AluI- or HinfI-digested bovine DNA with the (TG)n repeat used as a probe yielded substantial polymorphism. These results show that (i) different minisatellites can be found in a cluster, (ii) both simple and more complex repeated sequences other than the simple quaternary (GATA)n repeat can be sexually dimorphic, and (iii) simple repeats can reveal substantial polymorphism.
Mots-clé
Animals
Bacteriophage lambda/genetics
Base Sequence
Blotting, Southern
Cattle
Cloning, Molecular
DNA, Satellite/genetics
Female
Humans
Male
Molecular Sequence Data
Multigene Family
Nucleic Acid Hybridization
Polymorphism, Restriction Fragment Length
*Repetitive Sequences, Nucleic Acid
Restriction Mapping
*Sex Characteristics
Pubmed
Web of science
Création de la notice
25/01/2008 17:18
Dernière modification de la notice
20/08/2019 15:22