Article: article from journal or magazin.
A trap system for the isolation of amino acid sequences inducing GPI anchor addition.
Brazilian Journal of Medical and Biological Research
We designed a trap system to isolate different amino acid sequences which could target proteins to the cell surface via GPI anchor transfer. This selection procedure is based on the insertion of various sequences which regenerate a functional GPI anchor signal sequence and therefore provoke re-expression at the surface of a reporter molecule. Using this trap for cell surface targeting sequences, we could show the importance of the defined elements essential for GPI anchor addition. Such a system could be used for an exhaustive analysis of the carboxyl terminus structural requirements for GPI membrane anchoring.
Amino Acid Sequence, Antigens, Surface/chemistry, Antigens, Surface/metabolism, Cells, Cultured, Flow Cytometry, Genetic Vectors, Glycosylphosphatidylinositols/biosynthesis, Humans, Membrane Glycoproteins/chemistry, Membrane Glycoproteins/metabolism, Peptides/metabolism, Protein Sorting Signals, Transfection
Web of science
Last modification date