A trap system for the isolation of amino acid sequences inducing GPI anchor addition.

Détails

ID Serval
serval:BIB_64698EE58819
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
A trap system for the isolation of amino acid sequences inducing GPI anchor addition.
Périodique
Brazilian Journal of Medical and Biological Research
Auteur⸱e⸱s
Fasel N., Stark H., Bron C., Beghdadi-Rais C.
ISSN
0100-879X (Print)
ISSN-L
0100-879X
Statut éditorial
Publié
Date de publication
1994
Volume
27
Numéro
2
Pages
189-193
Langue
anglais
Résumé
We designed a trap system to isolate different amino acid sequences which could target proteins to the cell surface via GPI anchor transfer. This selection procedure is based on the insertion of various sequences which regenerate a functional GPI anchor signal sequence and therefore provoke re-expression at the surface of a reporter molecule. Using this trap for cell surface targeting sequences, we could show the importance of the defined elements essential for GPI anchor addition. Such a system could be used for an exhaustive analysis of the carboxyl terminus structural requirements for GPI membrane anchoring.
Mots-clé
Amino Acid Sequence, Antigens, Surface/chemistry, Antigens, Surface/metabolism, Cells, Cultured, Flow Cytometry, Genetic Vectors, Glycosylphosphatidylinositols/biosynthesis, Humans, Membrane Glycoproteins/chemistry, Membrane Glycoproteins/metabolism, Peptides/metabolism, Protein Sorting Signals, Transfection
Pubmed
Web of science
Création de la notice
24/01/2008 15:02
Dernière modification de la notice
20/08/2019 14:20
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