Peroxisome proliferator-activated receptor gamma is induced during differentiation of colon epithelium cells.

Details

Serval ID
serval:BIB_63F02BB15DBB
Type
Article: article from journal or magazin.
Collection
Publications
Title
Peroxisome proliferator-activated receptor gamma is induced during differentiation of colon epithelium cells.
Journal
Journal of Endocrinology
Author(s)
Lefebvre M., Paulweber B., Fajas L., Woods J., McCrary C., Colombel J.F., Najib J., Fruchart J.C., Datz C., Vidal H., Desreumaux P., Auwerx J.
ISSN
0022-0795 (Print)
ISSN-L
0022-0795
Publication state
Published
Issued date
1999
Volume
162
Number
3
Pages
331-340
Language
english
Abstract
Peroxisome proliferator-activated receptor gamma (PPARgamma), a fatty acid-activated nuclear receptor, is implicated in adipocyte differentiation and insulin sensitisation. In view of the association of dietary fat intake and bowel disease, the expression of PPARgamma in rodent and human intestine was studied. Expression of PPARgamma mRNA was examined by Northern blot hybridisation, RNase protection, and/or competitive RT-PCR assays, whereas PPARgamma protein levels were evaluated by immunoblotting and immunohistochemistry. PPARgamma mRNA and protein were abundantly expressed in colon relative to the small intestine both in rodents and in man. Interestingly, expression of PPARgamma was primarily localised in the more differentiated epithelial cells in the colon. The level of expression of PPARgamma in colon was similar to the levels seen in adipose tissue. Expression of PPARgamma increased from proximal to distal segments of the colon in man. In Caco-2 and HT-29 human adenocarcinoma cells, PPARgamma expression increased upon differentiation, consistent with PPARgamma being associated with a differentiated epithelial phenotype. High-level expression of PPARgamma was observed in the colon, but not in the small intestine, suggesting a potential role of this nuclear receptor in the colon.
Keywords
Aged, Animals, Blotting, Northern, Caco-2 Cells, Cell Differentiation, Cells, Cultured, Colon/chemistry, Epithelium/chemistry, HT29 Cells, Humans, Immunoblotting, Immunohistochemistry, Intestine, Small/chemistry, Male, Middle Aged, RNA, Messenger/analysis, Rats, Receptors, Cytoplasmic and Nuclear/analysis, Receptors, Cytoplasmic and Nuclear/genetics, Reverse Transcriptase Polymerase Chain Reaction, Transcription Factors/analysis, Transcription Factors/genetics
Pubmed
Web of science
Create date
07/03/2013 16:09
Last modification date
20/08/2019 14:20
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