Peroxisome proliferator-activated receptor gamma is induced during differentiation of colon epithelium cells.

Détails

ID Serval
serval:BIB_63F02BB15DBB
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Peroxisome proliferator-activated receptor gamma is induced during differentiation of colon epithelium cells.
Périodique
Journal of Endocrinology
Auteur⸱e⸱s
Lefebvre M., Paulweber B., Fajas L., Woods J., McCrary C., Colombel J.F., Najib J., Fruchart J.C., Datz C., Vidal H., Desreumaux P., Auwerx J.
ISSN
0022-0795 (Print)
ISSN-L
0022-0795
Statut éditorial
Publié
Date de publication
1999
Volume
162
Numéro
3
Pages
331-340
Langue
anglais
Résumé
Peroxisome proliferator-activated receptor gamma (PPARgamma), a fatty acid-activated nuclear receptor, is implicated in adipocyte differentiation and insulin sensitisation. In view of the association of dietary fat intake and bowel disease, the expression of PPARgamma in rodent and human intestine was studied. Expression of PPARgamma mRNA was examined by Northern blot hybridisation, RNase protection, and/or competitive RT-PCR assays, whereas PPARgamma protein levels were evaluated by immunoblotting and immunohistochemistry. PPARgamma mRNA and protein were abundantly expressed in colon relative to the small intestine both in rodents and in man. Interestingly, expression of PPARgamma was primarily localised in the more differentiated epithelial cells in the colon. The level of expression of PPARgamma in colon was similar to the levels seen in adipose tissue. Expression of PPARgamma increased from proximal to distal segments of the colon in man. In Caco-2 and HT-29 human adenocarcinoma cells, PPARgamma expression increased upon differentiation, consistent with PPARgamma being associated with a differentiated epithelial phenotype. High-level expression of PPARgamma was observed in the colon, but not in the small intestine, suggesting a potential role of this nuclear receptor in the colon.
Mots-clé
Aged, Animals, Blotting, Northern, Caco-2 Cells, Cell Differentiation, Cells, Cultured, Colon/chemistry, Epithelium/chemistry, HT29 Cells, Humans, Immunoblotting, Immunohistochemistry, Intestine, Small/chemistry, Male, Middle Aged, RNA, Messenger/analysis, Rats, Receptors, Cytoplasmic and Nuclear/analysis, Receptors, Cytoplasmic and Nuclear/genetics, Reverse Transcriptase Polymerase Chain Reaction, Transcription Factors/analysis, Transcription Factors/genetics
Pubmed
Web of science
Création de la notice
07/03/2013 16:09
Dernière modification de la notice
20/08/2019 14:20
Données d'usage