Coat color dilution in mice because of inactivation of the melanoma antigen MART-1.
Details
State: Public
Version: Author's accepted manuscript
Serval ID
serval:BIB_6214CD4B88BA
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Coat color dilution in mice because of inactivation of the melanoma antigen MART-1.
Journal
Pigment Cell and Melanoma Research
ISSN
1755-148X (Electronic)
ISSN-L
1755-1471
Publication state
Published
Issued date
10/2012
Peer-reviewed
Oui
Volume
25
Number
1
Pages
37-46
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Abstract
Melanoma antigen recognized by T cells 1 (MART-1) is a melanoma-specific antigen, which has been thoroughly studied in the context of immunotherapy against malignant melanoma and which is found only in the pigment cell lineage. However, its exact function and involvement in pigmentation is not clearly understood. Melanoma antigen recognized by T cells 1 has been shown to interact with the melanosomal proteins Pmel17 and OA1. To understand the function of MART-1 in pigmentation, we developed a new knockout mouse model. Mice deficient in MART-1 are viable, but loss of MART-1 leads to a coat color phenotype, with a reduction in total melanin content of the skin and hair. Lack of MART-1 did not affect localization of melanocyte-specific proteins nor maturation of Pmel17. Melanosomes of hair follicle melanocytes in MART-1 knockout mice displayed morphological abnormalities, which were exclusive to stage III and IV melanosomes. In conclusion, our results suggest that MART-1 is a pigmentation gene that is required for melanosome biogenesis and/or maintenance.
Keywords
Animals, Cells, Cultured/metabolism, Genetic Vectors, Hair/metabolism, Hair Color/genetics, MART-1 Antigen/genetics, MART-1 Antigen/physiology, Melanins/metabolism, Melanocytes/metabolism, Melanosomes/metabolism, Melanosomes/pathology, Mice, Mice, Inbred C57BL, Mice, Knockout, Phenotype, Skin/cytology, Skin/metabolism, gp100 Melanoma Antigen/metabolism
Pubmed
Web of science
Create date
28/06/2012 18:58
Last modification date
20/10/2020 11:08