Article: article from journal or magazin.
Cell uncoupling and protein kinase C: correlation in a cell line but not in a differentiated tissue
American Journal of Physiology
5 Pt 1
In Vitro Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. --- Old month value: Nov
Second messengers have been implicated in the control of communication between cells of various tissues and of a number of cell lines. To assess whether protein kinase C (PKC) is involved in the regulation of gap junctions between primary differentiated cells, we studied the effects of 12-O-tetradecanoylphorbol-13-acetate (TPA) on PKC translocation and junctional conductance of rat pancreatic exocrine cells. Our results show that although TPA induced the translocation of PKC from a "cytosolic" to a "microsomal" fraction within minutes, it failed to block the junctional conductance of acinar cell pairs up to 30 min after application. By contrast, analogous experiments on a liver-derived cell line (WB cells) showed that TPA-induced PKC translocation was paralleled by a marked and irreversible inhibition of intercellular coupling. These results indicate that, in contrast to the effects on transformed or dedifferentiated permanent cell lines, PKC is not involved in gating gap junctional channels between primary differentiated secretory cells of the pancreas.
Animals Cell Line Enzyme Activation/drug effects Intercellular Junctions/drug effects/*physiology Liver/cytology Male Pancreas/cytology Protein Kinase C/*physiology Rats Rats, Inbred Strains Tetradecanoylphorbol Acetate/pharmacology
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