Cell uncoupling and protein kinase C: correlation in a cell line but not in a differentiated tissue

Détails

ID Serval
serval:BIB_5F2E53132C8B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Cell uncoupling and protein kinase C: correlation in a cell line but not in a differentiated tissue
Périodique
American Journal of Physiology
Auteur(s)
Chanson  M., Bruzzone  R., Spray  D. C., Regazzi  R., Meda  P.
ISSN
0002-9513 (Print)
0363-6143
ISSN-L
0363-6143
Statut éditorial
Publié
Date de publication
11/1988
Volume
255
Numéro
5 Pt 1
Pages
C699-704
Notes
In Vitro Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. --- Old month value: Nov
Résumé
Second messengers have been implicated in the control of communication between cells of various tissues and of a number of cell lines. To assess whether protein kinase C (PKC) is involved in the regulation of gap junctions between primary differentiated cells, we studied the effects of 12-O-tetradecanoylphorbol-13-acetate (TPA) on PKC translocation and junctional conductance of rat pancreatic exocrine cells. Our results show that although TPA induced the translocation of PKC from a "cytosolic" to a "microsomal" fraction within minutes, it failed to block the junctional conductance of acinar cell pairs up to 30 min after application. By contrast, analogous experiments on a liver-derived cell line (WB cells) showed that TPA-induced PKC translocation was paralleled by a marked and irreversible inhibition of intercellular coupling. These results indicate that, in contrast to the effects on transformed or dedifferentiated permanent cell lines, PKC is not involved in gating gap junctional channels between primary differentiated secretory cells of the pancreas.
Mots-clé
Animals Cell Line Enzyme Activation/drug effects Intercellular Junctions/drug effects/*physiology Liver/cytology Male Pancreas/cytology Protein Kinase C/*physiology Rats Rats, Inbred Strains Tetradecanoylphorbol Acetate/pharmacology
Pubmed
Web of science
Création de la notice
24/01/2008 15:30
Dernière modification de la notice
20/08/2019 15:16
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