Acidic pH of the lateral intercellular spaces of MDCK cells cultured on permeable supports

Details

Serval ID
serval:BIB_5C337DDD0B82
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Acidic pH of the lateral intercellular spaces of MDCK cells cultured on permeable supports
Journal
Journal of Membrane Biology
Author(s)
Chatton  J. Y., Spring  K. R.
ISSN
0022-2631 (Print)
Publication state
Published
Issued date
06/1994
Volume
140
Number
2
Pages
89-99
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jun
Abstract
The pH of the lateral intercellular space (LIS) of Madin-Darby canine kidney (MDCK) cell monolayers grown on permeable supports was investigated by microspectrofluorimetry using BCECF (2',7'-bis(carboxyethyl)-5,6-carboxyfluorescein). The permeability of the support was selectively reduced by growing Zn-Al-silicate crystals inside its pores. The diffusion of BCECF across the filter was sufficiently retarded to allow measurements of fluorescence in the LIS. The LIS pH and intracellular pH of the cells surrounding them were determined in HEPES-buffered solutions. When the perfusate pH was 7.4, the LIS pH was more acidic (7.06 +/- 0.02) and equaled the cytoplasmic pH (7.08 +/- 0.05). When perfusate was changed to pH 7.0 or 7.8, the LIS changed linearly by about half the magnitude of the perfusate pH. Intracellular pH followed LIS pH variations between perfusate pH 7.0 and 7.4 but was significantly higher when perfusate pH was 7.8. Tight junctional H+ permeability was undetectably low. The low steady-state pH in the LIS was not altered by inhibitors of acid transport or low temperature. Rapid perturbations of pH in the LIS showed that protons were not immobilized in the LIS. The acidic microenvironment within the LIS may be the result of buffering by the cell surface proteins.
Keywords
Animals Biological Transport, Active Cell Line Cold Cytological Techniques Diffusion Dogs Extracellular Space/*metabolism Fluoresceins Hydrogen-Ion Concentration Intracellular Fluid/metabolism Kidney/metabolism Permeability
Pubmed
Web of science
Create date
24/01/2008 13:08
Last modification date
20/08/2019 14:14
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