Acidic pH of the lateral intercellular spaces of MDCK cells cultured on permeable supports

Détails

ID Serval
serval:BIB_5C337DDD0B82
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Acidic pH of the lateral intercellular spaces of MDCK cells cultured on permeable supports
Périodique
Journal of Membrane Biology
Auteur⸱e⸱s
Chatton  J. Y., Spring  K. R.
ISSN
0022-2631 (Print)
Statut éditorial
Publié
Date de publication
06/1994
Volume
140
Numéro
2
Pages
89-99
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jun
Résumé
The pH of the lateral intercellular space (LIS) of Madin-Darby canine kidney (MDCK) cell monolayers grown on permeable supports was investigated by microspectrofluorimetry using BCECF (2',7'-bis(carboxyethyl)-5,6-carboxyfluorescein). The permeability of the support was selectively reduced by growing Zn-Al-silicate crystals inside its pores. The diffusion of BCECF across the filter was sufficiently retarded to allow measurements of fluorescence in the LIS. The LIS pH and intracellular pH of the cells surrounding them were determined in HEPES-buffered solutions. When the perfusate pH was 7.4, the LIS pH was more acidic (7.06 +/- 0.02) and equaled the cytoplasmic pH (7.08 +/- 0.05). When perfusate was changed to pH 7.0 or 7.8, the LIS changed linearly by about half the magnitude of the perfusate pH. Intracellular pH followed LIS pH variations between perfusate pH 7.0 and 7.4 but was significantly higher when perfusate pH was 7.8. Tight junctional H+ permeability was undetectably low. The low steady-state pH in the LIS was not altered by inhibitors of acid transport or low temperature. Rapid perturbations of pH in the LIS showed that protons were not immobilized in the LIS. The acidic microenvironment within the LIS may be the result of buffering by the cell surface proteins.
Mots-clé
Animals Biological Transport, Active Cell Line Cold Cytological Techniques Diffusion Dogs Extracellular Space/*metabolism Fluoresceins Hydrogen-Ion Concentration Intracellular Fluid/metabolism Kidney/metabolism Permeability
Pubmed
Web of science
Création de la notice
24/01/2008 14:08
Dernière modification de la notice
20/08/2019 15:14
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