Article: article from journal or magazin.
Lentiviral-mediated gene transfer of siRNAs for the treatment of Huntington's disease.
Methods in Molecular Biology
Publication types: Journal ArticlePublication Status: ppublish
This chapter describes the potential use of viral-mediated gene transfer in the central nervous system for the silencing of gene expression using RNA interference in the context of Huntington's disease (HD). Protocols provided here describe the design of small interfering RNAs, their encoding in lentiviral vectors (LVs) and viral production, as well as procedures for their stereotaxic injection in the rodent brain.
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