Cycloheximide- and puromycin-induced heat resistance: different effects on cytoplasmic and nuclear luciferases.

Details

Serval ID
serval:BIB_57B766178042
Type
Article: article from journal or magazin.
Collection
Publications
Title
Cycloheximide- and puromycin-induced heat resistance: different effects on cytoplasmic and nuclear luciferases.
Journal
Cell Stress & Amp; Chaperones
Author(s)
Michels A.A., Kanon B., Konings A.W., Bensaude O., Kampinga H.H.
ISSN
1355-8145
Publication state
Published
Issued date
2000
Peer-reviewed
Oui
Volume
5
Number
3
Pages
181-187
Language
english
Abstract
Inhibition of translation can result in cytoprotection against heat shock. The mechanism of this protection has remained elusive so far. Here, the thermoprotective effects of the translation inhibitor cycloheximide (CHX) and puromycin were investigated, using as reporter firefly luciferase localized either in the nucleus or in the cytoplasm. A short preincubation of O23 cells with either translation inhibitor was found to attenuate the heat inactivation of a luciferase directed into the cytoplasm, whereas the heat sensitivity of a nuclear-targeted luciferase remained unaffected. After a long-term CHX pretreatment, both luciferases were more heat resistant. Both the cytoplasmic and the nuclear luciferase are protected against heat-induced inactivation in thermotolerant cells and in cells overexpressing heat shock protein (Hsp)70. CHX incubations further attenuated cytoplasmic luciferase inactivation in thermotolerant and in Hsp70 overexpressing cells, even when Hsp70-mediated protection was saturated. It is concluded that protection by translation inhibition is unlikely due to an increase in the pool of free Hsps normally engaged in translation and released from the nascent polypeptide chains on the ribosomes. Rather, a decrease in nascent chains and thermolabile polypeptides may account for the heat resistance promoted by inhibitors of translation.
Keywords
Animals, Cell Line, Cell Nucleus/enzymology, Cricetinae, Cycloheximide/pharmacology, Cytoplasm/enzymology, Cytoprotection, Enzyme Stability, Fibroblasts, Heat-Shock Response, Humans, Luciferases/genetics, Luciferases/metabolism, Plasmids/genetics, Plasmids/metabolism, Protein Synthesis Inhibitors/pharmacology, Puromycin/pharmacology, Temperature, Transfection
Pubmed
Web of science
Create date
28/07/2008 16:06
Last modification date
20/08/2019 14:11
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