Cycloheximide- and puromycin-induced heat resistance: different effects on cytoplasmic and nuclear luciferases.

Détails

ID Serval
serval:BIB_57B766178042
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Cycloheximide- and puromycin-induced heat resistance: different effects on cytoplasmic and nuclear luciferases.
Périodique
Cell Stress & Amp; Chaperones
Auteur⸱e⸱s
Michels A.A., Kanon B., Konings A.W., Bensaude O., Kampinga H.H.
ISSN
1355-8145
Statut éditorial
Publié
Date de publication
2000
Peer-reviewed
Oui
Volume
5
Numéro
3
Pages
181-187
Langue
anglais
Résumé
Inhibition of translation can result in cytoprotection against heat shock. The mechanism of this protection has remained elusive so far. Here, the thermoprotective effects of the translation inhibitor cycloheximide (CHX) and puromycin were investigated, using as reporter firefly luciferase localized either in the nucleus or in the cytoplasm. A short preincubation of O23 cells with either translation inhibitor was found to attenuate the heat inactivation of a luciferase directed into the cytoplasm, whereas the heat sensitivity of a nuclear-targeted luciferase remained unaffected. After a long-term CHX pretreatment, both luciferases were more heat resistant. Both the cytoplasmic and the nuclear luciferase are protected against heat-induced inactivation in thermotolerant cells and in cells overexpressing heat shock protein (Hsp)70. CHX incubations further attenuated cytoplasmic luciferase inactivation in thermotolerant and in Hsp70 overexpressing cells, even when Hsp70-mediated protection was saturated. It is concluded that protection by translation inhibition is unlikely due to an increase in the pool of free Hsps normally engaged in translation and released from the nascent polypeptide chains on the ribosomes. Rather, a decrease in nascent chains and thermolabile polypeptides may account for the heat resistance promoted by inhibitors of translation.
Mots-clé
Animals, Cell Line, Cell Nucleus/enzymology, Cricetinae, Cycloheximide/pharmacology, Cytoplasm/enzymology, Cytoprotection, Enzyme Stability, Fibroblasts, Heat-Shock Response, Humans, Luciferases/genetics, Luciferases/metabolism, Plasmids/genetics, Plasmids/metabolism, Protein Synthesis Inhibitors/pharmacology, Puromycin/pharmacology, Temperature, Transfection
Pubmed
Web of science
Création de la notice
28/07/2008 16:06
Dernière modification de la notice
20/08/2019 14:11
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