Article: article from journal or magazin.
Malignant peripheral nerve sheath tumors are t(X;18)-negative sarcomas. Molecular analysis of 25 cases occurring in neurofibromatosis type 1 patients, using two different RT-PCR-based methods of detection
PT - Journal Article PT - Research Support, Non-U.S. Gov't
To verify the absence of the synovial sarcoma translocation t(X;18) (SYT-SSX) in malignant peripheral nerve sheath tumors, 34 tumor samples from 25 neurofibromatosis type 1 patients were examined in two independent laboratories (Bordeaux, France, and Lausanne, Switzerland) using reverse transcriptase polymerase chain reaction (RT-PCR)-based techniques. RNA was extracted from paraffin blocks using standard methods, reverse transcribed, and conventional (in one laboratory) versus real-time (in the other laboratory) PCR performed. Twenty-seven tumor samples from 19 patients were negative for the t(X;18) in both laboratories; six additional tumors that were t(X;18)-negative in one laboratory gave noninterpretable results in the other, due to lack of internal positive controls; one case was noninterpretable in both places. In conclusion, malignant peripheral nerve sheath tumors in neurofibromatosis type 1 patients do not bear the synovial sarcoma t(X;18) (SYT-SSX). Laboratories that use PCR-based techniques for diagnostic purposes would benefit from quality assurance programs
Adolescent/Adult/Aged/Chromosomes,Human,Pair 18/genetics/Female/Humans/Male/Middle Aged/Nerve Sheath Neoplasms/complications/Pathology/Neurofibromatosis 1/Oncogene Proteins,Fusion/RNA,Neoplasm/metabolism/Reverse Transcriptase Polymerase Chain Reaction/Translocation,Genetic/X Chromosome
Web of science
Last modification date