Malignant peripheral nerve sheath tumors are t(X;18)-negative sarcomas. Molecular analysis of 25 cases occurring in neurofibromatosis type 1 patients, using two different RT-PCR-based methods of detection

Détails

ID Serval
serval:BIB_550B0655D37D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Malignant peripheral nerve sheath tumors are t(X;18)-negative sarcomas. Molecular analysis of 25 cases occurring in neurofibromatosis type 1 patients, using two different RT-PCR-based methods of detection
Périodique
Modern Pathology
Auteur(s)
Coindre  J. M., Hostein  I., Benhattar  J., Lussan  C., Rivel  J., Guillou  L.
ISSN
0893-3952 (Print)
Statut éditorial
Publié
Date de publication
2002
Volume
15
Numéro
6
Pages
589-592
Notes
PT - Journal Article PT - Research Support, Non-U.S. Gov't
Résumé
To verify the absence of the synovial sarcoma translocation t(X;18) (SYT-SSX) in malignant peripheral nerve sheath tumors, 34 tumor samples from 25 neurofibromatosis type 1 patients were examined in two independent laboratories (Bordeaux, France, and Lausanne, Switzerland) using reverse transcriptase polymerase chain reaction (RT-PCR)-based techniques. RNA was extracted from paraffin blocks using standard methods, reverse transcribed, and conventional (in one laboratory) versus real-time (in the other laboratory) PCR performed. Twenty-seven tumor samples from 19 patients were negative for the t(X;18) in both laboratories; six additional tumors that were t(X;18)-negative in one laboratory gave noninterpretable results in the other, due to lack of internal positive controls; one case was noninterpretable in both places. In conclusion, malignant peripheral nerve sheath tumors in neurofibromatosis type 1 patients do not bear the synovial sarcoma t(X;18) (SYT-SSX). Laboratories that use PCR-based techniques for diagnostic purposes would benefit from quality assurance programs
Mots-clé
Adolescent/Adult/Aged/Chromosomes,Human,Pair 18/genetics/Female/Humans/Male/Middle Aged/Nerve Sheath Neoplasms/complications/Pathology/Neurofibromatosis 1/Oncogene Proteins,Fusion/RNA,Neoplasm/metabolism/Reverse Transcriptase Polymerase Chain Reaction/Translocation,Genetic/X Chromosome
Pubmed
Web of science
Open Access
Oui
Création de la notice
29/01/2008 19:35
Dernière modification de la notice
20/08/2019 15:09
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