Usefulness of double locus sequence typing (DLST) for regional and international epidemiological surveillance of methicilin-resistant Staphylococcus aureus.

Details

Serval ID
serval:BIB_52DCE299B052
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Usefulness of double locus sequence typing (DLST) for regional and international epidemiological surveillance of methicilin-resistant Staphylococcus aureus.
Journal
Clinical Microbiology and Infection
Author(s)
Basset P., Senn L., Prod'hom G., Bille J., Francioli P., Zanetti G., Blanc D.S.
ISSN
1198-743X[linking]
ISSN-L
1469-0691[electronic],
Publication state
Published
Issued date
2010
Peer-reviewed
Oui
Volume
16
Number
8
Pages
1289-1296
Language
english
Notes
Publication types: Comparative Study ; Evaluation Studies ; Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Abstract
Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of nosocomial infections worldwide. To differentiate reliably among S. aureus isolates, we recently developed double locus sequence typing (DLST) based on the analysis of partial sequences of clfB and spa genes. In the present study, we evaluated the usefulness of DLST for epidemiological investigations of MRSA by routinely typing 1242 strains isolated in Western Switzerland. Additionally, particular local and international collections were typed by pulsed field gel electrophoresis (PFGE) and DLST to check the compatibility of DLST with the results obtained by PFGE, and for international comparisons. Using DLST, we identified the major MRSA clones of Western Switzerland, and demonstrated the close relationship between local and international clones. The congruence of 88% between the major PFGE and DLST clones indicated that our results obtained by DLST were compatible with earlier results obtained by PFGE. DLST could thus easily be incorporated in a routine surveillance procedure. In addition, the unambiguous definition of DLST types makes this method more suitable than PFGE for long-term epidemiological surveillance. Finally, the comparison of the results obtained by DLST, multilocus sequence typing, PFGE, Staphylococcal cassette chromosome mec typing and the detection of Panton-Valentine leukocidin genes indicated that no typing scheme should be used on its own. It is only the combination of data from different methods that gives the best chance of describing precisely the epidemiology and phylogeny of MRSA.
Keywords
Bacterial Proteins/genetics, Bacterial Typing Techniques, Cluster Analysis, Cross Infection/epidemiology, Cross Infection/microbiology, DNA Fingerprinting, Electrophoresis, Gel, Pulsed-Field, Genotype, Humans, Methicillin-Resistant Staphylococcus aureus/classification, Methicillin-Resistant Staphylococcus aureus/genetics, Molecular Epidemiology/methods, Sequence Analysis, DNA/methods, Staphylococcal Infections/epidemiology, Staphylococcal Infections/microbiology, Type="Geographic">Switzerland/epidemiology
Pubmed
Web of science
Open Access
Yes
Create date
30/12/2010 15:31
Last modification date
20/08/2019 14:08
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