Usefulness of double locus sequence typing (DLST) for regional and international epidemiological surveillance of methicilin-resistant Staphylococcus aureus.

Détails

ID Serval
serval:BIB_52DCE299B052
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Usefulness of double locus sequence typing (DLST) for regional and international epidemiological surveillance of methicilin-resistant Staphylococcus aureus.
Périodique
Clinical Microbiology and Infection
Auteur⸱e⸱s
Basset P., Senn L., Prod'hom G., Bille J., Francioli P., Zanetti G., Blanc D.S.
ISSN
1198-743X[linking]
ISSN-L
1469-0691[electronic],
Statut éditorial
Publié
Date de publication
2010
Peer-reviewed
Oui
Volume
16
Numéro
8
Pages
1289-1296
Langue
anglais
Notes
Publication types: Comparative Study ; Evaluation Studies ; Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Résumé
Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of nosocomial infections worldwide. To differentiate reliably among S. aureus isolates, we recently developed double locus sequence typing (DLST) based on the analysis of partial sequences of clfB and spa genes. In the present study, we evaluated the usefulness of DLST for epidemiological investigations of MRSA by routinely typing 1242 strains isolated in Western Switzerland. Additionally, particular local and international collections were typed by pulsed field gel electrophoresis (PFGE) and DLST to check the compatibility of DLST with the results obtained by PFGE, and for international comparisons. Using DLST, we identified the major MRSA clones of Western Switzerland, and demonstrated the close relationship between local and international clones. The congruence of 88% between the major PFGE and DLST clones indicated that our results obtained by DLST were compatible with earlier results obtained by PFGE. DLST could thus easily be incorporated in a routine surveillance procedure. In addition, the unambiguous definition of DLST types makes this method more suitable than PFGE for long-term epidemiological surveillance. Finally, the comparison of the results obtained by DLST, multilocus sequence typing, PFGE, Staphylococcal cassette chromosome mec typing and the detection of Panton-Valentine leukocidin genes indicated that no typing scheme should be used on its own. It is only the combination of data from different methods that gives the best chance of describing precisely the epidemiology and phylogeny of MRSA.
Mots-clé
Bacterial Proteins/genetics, Bacterial Typing Techniques, Cluster Analysis, Cross Infection/epidemiology, Cross Infection/microbiology, DNA Fingerprinting, Electrophoresis, Gel, Pulsed-Field, Genotype, Humans, Methicillin-Resistant Staphylococcus aureus/classification, Methicillin-Resistant Staphylococcus aureus/genetics, Molecular Epidemiology/methods, Sequence Analysis, DNA/methods, Staphylococcal Infections/epidemiology, Staphylococcal Infections/microbiology, Type="Geographic">Switzerland/epidemiology
Pubmed
Web of science
Open Access
Oui
Création de la notice
30/12/2010 16:31
Dernière modification de la notice
20/08/2019 15:08
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