A phosphorylation cycle shapes gradients of the DYRK family kinase Pom1 at the plasma membrane.

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Ressource 1Télécharger: BIB_523F78C4557D.P001.pdf (4000.99 [Ko])
Etat: Serval
Version: Final published version
ID Serval
serval:BIB_523F78C4557D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
A phosphorylation cycle shapes gradients of the DYRK family kinase Pom1 at the plasma membrane.
Périodique
Cell
Auteur(s)
Hachet O., Berthelot-Grosjean M., Kokkoris K., Vincenzetti V., Moosbrugger J., Martin S.G.
ISSN
1097-4172 (Electronic)
ISSN-L
0092-8674
Statut éditorial
Publié
Date de publication
2011
Peer-reviewed
Oui
Volume
145
Numéro
7
Pages
1116-1128
Langue
anglais
Résumé
Concentration gradients regulate many cell biological and developmental processes. In rod-shaped fission yeast cells, polar cortical gradients of the DYRK family kinase Pom1 couple cell length with mitotic commitment by inhibiting a mitotic inducer positioned at midcell. However, how Pom1 gradients are established is unknown. Here, we show that Tea4, which is normally deposited at cell tips by microtubules, is both necessary and, upon ectopic cortical localization, sufficient to recruit Pom1 to the cell cortex. Pom1 then moves laterally at the plasma membrane, which it binds through a basic region exhibiting direct lipid interaction. Pom1 autophosphorylates in this region to lower lipid affinity and promote membrane release. Tea4 triggers Pom1 plasma membrane association by promoting its dephosphorylation through the protein phosphatase 1 Dis2. We propose that local dephosphorylation induces Pom1 membrane association and nucleates a gradient shaped by the opposing actions of lateral diffusion and autophosphorylation-dependent membrane detachment.
Mots-clé
Amino Acid Sequence, Cell Cycle, Cell Membrane/metabolism, Microtubule-Associated Proteins/metabolism, Molecular Sequence Data, Phosphorylation, Protein Kinases/chemistry, Protein Kinases/metabolism, Schizosaccharomyces/cytology, Schizosaccharomyces/metabolism, Schizosaccharomyces pombe Proteins/metabolism, Sequence Alignment
Pubmed
Web of science
Open Access
Oui
Création de la notice
12/10/2011 13:46
Dernière modification de la notice
08/05/2019 18:37
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