Design and synthesis of a TICT-based red-emissive fluorescent probe for the rapid and selective detection of HSA in human biofluids and live cell imaging.

Details

Serval ID
serval:BIB_523510B3DDCF
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Design and synthesis of a TICT-based red-emissive fluorescent probe for the rapid and selective detection of HSA in human biofluids and live cell imaging.
Journal
Journal of materials chemistry. B
Author(s)
Moni D., Sasmal M., Katarkar A., Basu A., Ali M.
ISSN
2050-7518 (Electronic)
ISSN-L
2050-750X
Publication state
Published
Issued date
11/09/2024
Peer-reviewed
Oui
Volume
12
Number
35
Pages
8791-8800
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Here, we report the design and synthesis of a D⋯π⋯A-based fluorescent probe, (E)-4-(4-(dibutylamine)-2-hydroxystyryl)-1-methylquinolin-1-ium (DHMQ), which is nonfluorescent in ∼100% PBS buffer medium due to a twisted intra molecular charge transfer (TICT) phenomenon and it becomes highly fluorescent (∼149 fold) in the presence of human serum albumin (HSA), owing to the restriction of its intramolecular free rotation inside the hydrophobic binding cavity of HSA. The site-selective fluorescence displacement assay and molecular docking studies clearly reveal that DHMQ selectively binds at subdomain IB of HSA. The 3σ/slope method was adopted to determine the limit of detection (LOD) value, which was as low as 2.39 nM in ∼100% PBS medium, indicating its high sensitivity towards HSA. The low dissociation constant value [K <sub>d</sub> = (1.066 ± 0.017) μM] suggests a strong complexation between the DHMQ and HSA. Importantly, it has been demonstrated that DHMQ is capable of detecting HSA in real human serum and urine samples and was found to be suitable for live cell imaging of HSA.
Keywords
Humans, Fluorescent Dyes/chemistry, Fluorescent Dyes/chemical synthesis, Serum Albumin, Human/analysis, Serum Albumin, Human/metabolism, Molecular Docking Simulation, Molecular Structure, Optical Imaging
Pubmed
Web of science
Create date
19/08/2024 12:46
Last modification date
14/09/2024 6:12
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