Design and synthesis of a TICT-based red-emissive fluorescent probe for the rapid and selective detection of HSA in human biofluids and live cell imaging.
Détails
ID Serval
serval:BIB_523510B3DDCF
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Design and synthesis of a TICT-based red-emissive fluorescent probe for the rapid and selective detection of HSA in human biofluids and live cell imaging.
Périodique
Journal of materials chemistry. B
ISSN
2050-7518 (Electronic)
ISSN-L
2050-750X
Statut éditorial
Publié
Date de publication
11/09/2024
Peer-reviewed
Oui
Volume
12
Numéro
35
Pages
8791-8800
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Publication Status: epublish
Résumé
Here, we report the design and synthesis of a D⋯π⋯A-based fluorescent probe, (E)-4-(4-(dibutylamine)-2-hydroxystyryl)-1-methylquinolin-1-ium (DHMQ), which is nonfluorescent in ∼100% PBS buffer medium due to a twisted intra molecular charge transfer (TICT) phenomenon and it becomes highly fluorescent (∼149 fold) in the presence of human serum albumin (HSA), owing to the restriction of its intramolecular free rotation inside the hydrophobic binding cavity of HSA. The site-selective fluorescence displacement assay and molecular docking studies clearly reveal that DHMQ selectively binds at subdomain IB of HSA. The 3σ/slope method was adopted to determine the limit of detection (LOD) value, which was as low as 2.39 nM in ∼100% PBS medium, indicating its high sensitivity towards HSA. The low dissociation constant value [K <sub>d</sub> = (1.066 ± 0.017) μM] suggests a strong complexation between the DHMQ and HSA. Importantly, it has been demonstrated that DHMQ is capable of detecting HSA in real human serum and urine samples and was found to be suitable for live cell imaging of HSA.
Mots-clé
Humans, Fluorescent Dyes/chemistry, Fluorescent Dyes/chemical synthesis, Serum Albumin, Human/analysis, Serum Albumin, Human/metabolism, Molecular Docking Simulation, Molecular Structure, Optical Imaging
Pubmed
Web of science
Création de la notice
19/08/2024 12:46
Dernière modification de la notice
14/09/2024 6:12