Rab3a controls exocytosis in cholecystokinin-secreting cells

Détails

ID Serval
serval:BIB_51411913FB25
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Rab3a controls exocytosis in cholecystokinin-secreting cells
Périodique
FEBS Letters
Auteur(s)
Gevrey  J. C., Laurent  S., Saurin  J. C., Nemoz-Gaillard  E., Regazzi  R., Chevrier  A. M., Chayvialle  J. A., Abello  J.
ISSN
0014-5793 (Print)
Statut éditorial
Publié
Date de publication
08/2001
Volume
503
Numéro
1
Pages
19-24
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Aug 10
Résumé
The expression of rab3A and rab3D isoforms in the enteroendocrine, cholecystokinin-secreting, cell lines STC-1 and GLUTag is here demonstrated. In contrast, rab3B is undetectable in these two cell lines, and rab3C is only slightly expressed in GLUTag cells. Using a transient co-transfection system with human growth hormone as reporter protein, we show that overexpression of the GTPase-deficient mutant rab3AQ81L, but not rab3DQ81L, significantly decreases human growth hormone secretory responses to various agonists in STC-1 cells. These results indicate that endocrine cell lines of intestinal origin express rab3A and rab3D proteins, but the GTP-bound form of rab3A only acts as a negative modulator in the control of cholecystokinin secretion from STC-1 cells.
Mots-clé
Animals Cell Line Cholecystokinin/*secretion Exocytosis/*physiology Genes, Reporter Growth Hormone/genetics Immunohistochemistry Mice Mutation Rats Transfection rab3A GTP-Binding Protein/genetics/*physiology
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 15:29
Dernière modification de la notice
08/05/2019 18:34
Données d'usage